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20231223083224.0 |
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231223s2006 xx ||||| 00| ||eng c |
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|a (NLM)16291801
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Tesniere, Catherine
|e verfasserin
|4 aut
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|a Effects of genetic manipulation of alcohol dehydrogenase levels on the response to stress and the synthesis of secondary metabolites in grapevine leaves
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|c 2006
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 06.04.2006
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|a Date Revised 15.11.2006
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The functional role of Adh in regulating susceptibility to abiotic stress and the synthesis of secondary metabolites was investigated in transgenic grapevine plants over- and underexpressing alcohol dehydrogenase (Adh). Plants were transformed with gene constructs containing a sense or antisense orientated grapevine VvAdh2 cDNA under the constitutive cauliflower mosaic virus 35S promoter. Plants transformed with either antisense orientation or the Adh-less construct displayed a low but detectable constitutive ADH activity, whereas plants transformed with the sense-expressed transgene showed a significantly higher (100-fold) ADH activity than the control. Compared with the control, the sense transgene induced an overexpression of VvAdh2 transcripts, whereas a reduced VvAdh2 expression was detected in antisense transformants. Grapevine plants overexpressing Adh displayed a lower sucrose content, a higher degree of polymerization of proanthocyanidins, and a generally increased content of volatile compounds, mainly in carotenoid- and shikimate-derived volatiles. In general, no significant differences between sense/antisense transformants were observed with regard to carotenoid and chlorophyll contents, suggesting a strong metabolic regulation of the synthesis of these compounds
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Coumaric Acids
|2 NLM
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|a Flavonols
|2 NLM
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|a Hexoses
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|a Norisoprenoids
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|a Proanthocyanidins
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|a Terpenes
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|a Chlorophyll
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|a 1406-65-1
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|a Carotenoids
|2 NLM
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|a 36-88-4
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|a Alcohol Dehydrogenase
|2 NLM
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|a EC 1.1.1.1
|2 NLM
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|a Torregrosa, Laurent
|e verfasserin
|4 aut
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|a Pradal, Martine
|e verfasserin
|4 aut
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|a Souquet, Jean-Marc
|e verfasserin
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|a Gilles, Christine
|e verfasserin
|4 aut
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|a Dos Santos, Karine
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|4 aut
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|a Chatelet, Philippe
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|4 aut
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|a Gunata, Ziya
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 57(2006), 1 vom: 15., Seite 91-9
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:57
|g year:2006
|g number:1
|g day:15
|g pages:91-9
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|d 57
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|h 91-9
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