In vivo imaging of MADS-box transcription factor interactions

MADS-box transcription factors are major regulators of development in flowering plants. The factors act in a combinatorial manner, either as homo- or heterodimers, and they control floral organ formation and identity and many other developmental processes through a complex network of protein-protein...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 57(2006), 1 vom: 15., Seite 33-42
1. Verfasser: Tonaco, Isabella A Nougalli (VerfasserIn)
Weitere Verfasser: Borst, Jan Willem, de Vries, Sacco C, Angenent, Gerco C, Immink, Richard G H
Format: Aufsatz
Sprache:English
Veröffentlicht: 2006
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't MADS Domain Proteins Multiprotein Complexes Plant Proteins
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520 |a MADS-box transcription factors are major regulators of development in flowering plants. The factors act in a combinatorial manner, either as homo- or heterodimers, and they control floral organ formation and identity and many other developmental processes through a complex network of protein-protein and protein-DNA interactions. Despite the fact that many studies have been carried out to elucidate MADS-box protein dimerization by yeast systems, very little information is available on the behaviour of these molecules in planta. Here, evidence for specific interactions between the petunia MADS-box proteins FBP2, FBP11, and FBP24 is provided in vivo. The dimers identified in yeast for the ovule-specific FBP24 protein have been confirmed in living plant cells by means of fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy and, in addition, some of the most likely, less stable homo- and heterodimers were identified. This in vivo approach revealed that particular dimers could only be detected in specific sub-nuclear domains. In addition, evidence for the in planta assembly of these ovule-specific MADS-box transcription factors into higher-order complexes is provided 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Multiprotein Complexes  |2 NLM 
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700 1 |a Borst, Jan Willem  |e verfasserin  |4 aut 
700 1 |a de Vries, Sacco C  |e verfasserin  |4 aut 
700 1 |a Angenent, Gerco C  |e verfasserin  |4 aut 
700 1 |a Immink, Richard G H  |e verfasserin  |4 aut 
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