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01000naa a22002652 4500 |
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NLM158955951 |
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DE-627 |
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20231223083206.0 |
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tu |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0530.xml
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|a (DE-627)NLM158955951
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|a (NLM)16290070
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zhang, Wei
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A peptide DNA surrogate that binds and inhibits anti-dsDNA antibodies
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| 264 |
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1 |
|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 16.02.2006
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|a Date Revised 14.11.2007
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Autoantibodies to dsDNA are an important diagnostic marker and pathogenic factor for systemic lupus erythematosus (SLE). Although the anti-dsDNA antibodies present in SLE are indicative of an antigen-driven response, the antigen has not been conclusively identified. The specific SLE anti-dsDNA antibodies were obtained by affinity purification using a dsDNA-coupled Sepharose column. Using the anti-dsDNA antibodies to screen a phage peptide display library, we demonstrated that purified polyclonal anti-dsDNA antibodies and a monoclonal anti-dsDNA antibody specifically bind a 15 mer peptide ASPVTARVLWKASHV. This chemically synthesized peptide could be recognized by anti-dsDNA antibodies in ELISA and Dot blot. This 15 mer peptide can inhibit anti-dsDNA antibodies binding to dsDNA antigen in immunoassays and in the Crithidia luciliae assay while a control peptide did not inhibit anti-dsDNA antibodies. This study demonstrates the potential usefulness of the peptide DNA surrogate in diagnostic tests of SLE and in the investigation of the origin of anti-dsDNA antibodies. It may also be used in studies of the DNA-anti-DNA antibody interaction
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, N.I.H., Extramural
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| 650 |
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4 |
|a Review
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| 650 |
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7 |
|a Antibodies, Antinuclear
|2 NLM
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| 650 |
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7 |
|a Immunoglobulin G
|2 NLM
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| 650 |
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7 |
|a Immunologic Factors
|2 NLM
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| 650 |
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|a Peptide Library
|2 NLM
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| 650 |
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|a Peptide Nucleic Acids
|2 NLM
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| 650 |
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7 |
|a Peptides
|2 NLM
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| 700 |
1 |
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|a Reichlin, Morris
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 117(2005), 3 vom: 15. Dez., Seite 214-20
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:117
|g year:2005
|g number:3
|g day:15
|g month:12
|g pages:214-20
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 117
|j 2005
|e 3
|b 15
|c 12
|h 214-20
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