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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0529.xml
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|a (DE-627)NLM158716434
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|a (NLM)16263905
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Leboeuf, Edouard
|e verfasserin
|4 aut
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| 245 |
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|a Biochemical and immunohistochemical analysis of pectic polysaccharides in the cell walls of Arabidopsis mutant QUASIMODO 1 suspension-cultured cells
|b implications for cell adhesion
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 20.01.2006
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|a Date Revised 03.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Mutation in the Arabidopsis thaliana QUASIMODO 1 gene (QUA1), which encodes a putative glycosyltransferase, reduces cell wall pectin content and cell adhesion. Suspension-cultured calli were generated from roots of wild-type (wt) and qua1-1 A. thaliana plants. The altered cell adhesion phenotype of the qua1-1 plant was also found with its suspension-cultured calli. Cell walls of both wt and qua1-1 calli were analysed by chemical, enzymatic and immunohistochemical techniques in order to assess the role of pectic polysaccharides in the mutant phenotype. Compared with the wt, qua1-1 calli cell walls contained more arabinose (23.6 versus 21.6 mol%), rhamnose (3.1 versus 2.7 mol%), and fucose (1.4 versus 1.2 mol%) and less uronic acid (24.2 versus 27.6 mol%), and they were less methyl-esterified (DM: 22.9% versus 30.3%). When sequential pectin extraction of calli cell walls was performed, qua1-1 water-soluble and chelator-soluble extracts contained more arabinose and less uronic acid than wt. Water-soluble pectins were less methyl-esterified in qua1-1 than in wt. Chelator-soluble pectins were more acetyl-esterified in qua1-1. Differences in the cell wall chemistry of wt and mutant calli were supported by a reduction in JIM7 labelling (methyl-esterified homogalacturonan) of the whole wall in small cells and particularly by a reduced labelling with 2F4 (calcium-associated homogalacturonan) in the middle lamella at tricellular junctions of large qua1-1 cells. Differences in the oligosaccharide profile obtained after endopolygalacturonase degradation of alkali extracts from qua1-1 and wt calli indicated variations in the structure of covalently bonded homogalacturonan. About 29% more extracellular polymers rich in pectins were recovered from the calli culture medium of qua1-1 compared with wt. These results show that perturbation of QUASIMODO 1-1 gene expression in calli resulted in alterations of homogalacturonan content and cell wall location. The consequences of these structural variations are discussed with regard to plant cell adhesion
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|a Journal Article
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|a Alkalies
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Chelating Agents
|2 NLM
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|a Culture Media
|2 NLM
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|a Polysaccharides
|2 NLM
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| 650 |
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|a Pectins
|2 NLM
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| 650 |
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|a 89NA02M4RX
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 650 |
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|a polygalacturonic acid
|2 NLM
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| 650 |
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|a VV3XD4CL04
|2 NLM
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| 700 |
1 |
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|a Guillon, Fabienne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thoiron, Séverine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lahaye, Marc
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 422 vom: 01. Dez., Seite 3171-82
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:56
|g year:2005
|g number:422
|g day:01
|g month:12
|g pages:3171-82
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|d 56
|j 2005
|e 422
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|h 3171-82
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