Facile means for quantifying microRNA expression by real-time PCR
MicroRNAs (miRNAs) are 20-24 nucleotide RNAs that are predicted to play regulatory roles in animals and plants. Here we report a simple and sensitive real-time PCR method for quantifying the expression of plant miRNAs. Total RNA, including miRNAs, was polyadenylated and reverse-transcribed with a po...
| Veröffentlicht in: | BioTechniques. - 1991. - 39(2005), 4 vom: 19. Okt., Seite 519-25 |
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| Format: | Aufsatz |
| Sprache: | English |
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2005
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, U.S. Gov't, Non-P.H.S. Benzothiazoles DNA Primers DNA, Complementary Diamines Fluorescent Dyes MicroRNAs Organic Chemicals Quinolines mehr... |