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231223s2004 xx ||||| 00| ||chi c |
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|a pubmed25n0528.xml
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|a (NLM)16221338
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Xu, Ke
|e verfasserin
|4 aut
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| 245 |
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|a Effect of Lp(a) on human mesangial cell proliferation, adhesion and migration
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1 |
|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 01.06.2010
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|a Date Revised 07.06.2016
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: The renal disease is commonly associated with hyperlipidemia and correlates with glomerular accumulation of atherogenic lipoproteins and mesangial hypercellularity. Therefore, in this study, the authors investigated a possible growth stimulatory effect and mode of action of lipoprotein(a) [Lp(a)] in human mesangial cells HMC, and the effect of Lp(a) on adhesion and migration in human mesangial cells
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|a METHODS: The DNA synthesis of HMC was measured by (3)H-thymidine incorporation. The cell adhesion was detected by the expression of vinculin by means of indirect immunofluorescence. The cell migration was observed under the microscope
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| 520 |
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|a RESULTS: The incubation of HMC with Lp(a) for 24 hours induced a significant dose-dependent proliferation of HMC [Lp(a): 5 microg, 10 microg, 25 microg, 50 microg/ml vs. control 0 microg/ml; (3)H-TdR incorporation (x 10(3)cpm): 1.69 +/- 0.48, 3.59 +/- 0.68, 4.14 +/- 0.78, 4.05 +/- 0.55 vs. 1.64 +/- 0.31, P < 0.01]. The vinculin staining by indirect immunofluorescence showed positive result when HMC was incubated with 10 microg/ml Lp(a) for 24 hours, while vinculin was negative when HMC was incubated with 0 microg/ml Lp(a) as the control of the study. The incubation of HMC with 10 microg/ml Lp(a) for 72 hours demonstrated significant cell migration effect compared to the control of 0 microg/ml. (16.2/LP vs. 2.4/LP, P < 0.01)
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|a CONCLUSION: Lp(a) could stimulate a proliferation, adhesion and migration effect on human mesangial cells
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| 650 |
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|a English Abstract
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| 650 |
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|a Journal Article
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| 650 |
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|a Intercellular Signaling Peptides and Proteins
|2 NLM
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| 650 |
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|a Lipoprotein(a)
|2 NLM
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| 700 |
1 |
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|a Song, Hong-mei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wei, Min
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhonghua er ke za zhi = Chinese journal of pediatrics
|d 1960
|g 42(2004), 10 vom: 13. Okt., Seite 734-6
|w (DE-627)NLM136249191
|x 0578-1310
|7 nnns
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| 773 |
1 |
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|g volume:42
|g year:2004
|g number:10
|g day:13
|g month:10
|g pages:734-6
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