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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0526.xml
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|a (DE-627)NLM157849244
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|a (NLM)16171363
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Hessler, Jessica A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Atomic force microscopy study of early morphological changes during apoptosis
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 17.04.2007
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|a Date Revised 03.12.2007
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|a published: Print
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|a Citation Status MEDLINE
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|a Apoptosis is defined by a distinct set of morphological changes observed during cell death including loss of focal adhesions, the formation of cell membrane buds or blebs, and a decrease in total cell volume. Recent studies suggest that these dramatic morphological changes, particularly apoptotic volume decrease (AVD), are an early prerequisite to apoptosis and precede key biochemical time-points. Here we use atomic force microscopy to observe early stage AVD of KB cells undergoing staurosporine-induced apoptosis. After a 3-h exposure to 1 microM staurosporine, a 32% decrease in total cell height and a 50% loss of total cell volume is observed accompanied by only a 15% change in cell diameter. The observed AVD precedes key biochemical hallmarks of apoptosis such as loss of mitochondrial membrane potential, phosphatidyl serine translocation, nuclear fragmentation, and measurable caspase-3 activity. This suggests that morphological volume changes occur very early in the induction of apoptosis
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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7 |
|a Phosphatidylserines
|2 NLM
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| 700 |
1 |
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|a Budor, Andrew
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Putchakayala, Krishna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mecke, Almut
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rieger, Daniel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Banaszak Holl, Mark M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Orr, Bradford G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bielinska, Anna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Beals, James
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baker, James
|c Jr
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 21(2005), 20 vom: 27. Sept., Seite 9280-6
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:21
|g year:2005
|g number:20
|g day:27
|g month:09
|g pages:9280-6
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a GBV_ILN_721
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|a AR
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| 952 |
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|d 21
|j 2005
|e 20
|b 27
|c 09
|h 9280-6
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