Large-scale fabrication of protein nanoarrays based on nanosphere lithography

Hexagonally patterned lysozyme nanoarrays have been assembled on silicon wafers by combining nanosphere lithography and surface silane chemistry using vapor and solution deposition processes. The patterned protein regions extend over cm sized regions, and the size of each island is approximately 120...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1991. - 21(2005), 20 vom: 27. Sept., Seite 9274-9
1. Verfasser: Cai, Yuguang (VerfasserIn)
Weitere Verfasser: Ocko, Benjamin M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Silanes Propylene Glycol 6DC9Q167V3 Muramidase EC 3.2.1.17 Trypsin EC 3.4.21.4 Silicon Z4152N8IUI
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520 |a Hexagonally patterned lysozyme nanoarrays have been assembled on silicon wafers by combining nanosphere lithography and surface silane chemistry using vapor and solution deposition processes. The patterned protein regions extend over cm sized regions, and the size of each island is approximately 120 nm for the solution-prepared template and approximately 60 nm for the vapor-prepared template. Antibody test indicates that the patterned lysozyme maintains its bioactivity on the surface. This new approach offers a fast and reliable method to fabricate protein arrays over large areas with feature sizes comparable to scanning-probe based techniques 
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