|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM157753646 |
| 003 |
DE-627 |
| 005 |
20231223080831.0 |
| 007 |
tu |
| 008 |
231223s2005 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0526.xml
|
| 035 |
|
|
|a (DE-627)NLM157753646
|
| 035 |
|
|
|a (NLM)16160751
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Timmermans, Martijn J T N
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Metallothionein mRNA expression and cadmium tolerance in metal-stressed and reference populations of the springtail Orchesella cincta
|
| 264 |
|
1 |
|c 2005
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 24.02.2006
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Metal contamination in soil ecosystems is a permanent and often strong selection pressure. The present study investigates metal tolerance in 17 Orchesella cincta (Collembola) populations from metal-contaminated and reference sites, and combines analyses at the phenotypic and molecular level. Metal tolerance was phenotypically assayed by measuring survival times of laboratory cultures during exposure to cadmium. Comparisons of survival curves showed that five out of eight metal-stressed populations tested evolved increased cadmium tolerance (Stolberg, Plombieres, Hoboken, Hygum and Gusum). In addition, the role of the metallothionein (MT) gene in cadmium tolerance of O. cincta was studied by means of quantitative RT-PCR. The constitutive and Cd-induced MT mRNA expression of the laboratory cultures was measured. Results show that the mean constitutive MT mRNA expression of populations from polluted sites was significantly higher than of populations from reference sites. However, no correlation between MT mRNA expression levels after laboratory exposure to cadmium and field cadmium concentrations was observed. Furthermore, no relation between survival rate during exposure to cadmium and MT mRNA expression was detected. Our results suggest that constitutive MT mRNA expression plays a role in early protection against cadmium toxicity, and indicate that mechanisms other then MT up-regulation are involved in tolerance to prolonged exposure to cadmium
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
7 |
|a Metals, Heavy
|2 NLM
|
| 650 |
|
7 |
|a RNA, Messenger
|2 NLM
|
| 650 |
|
7 |
|a Soil Pollutants
|2 NLM
|
| 650 |
|
7 |
|a Metallothionein
|2 NLM
|
| 650 |
|
7 |
|a 9038-94-2
|2 NLM
|
| 700 |
1 |
|
|a Ellers, Jacintha
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Roelofs, Dick
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a van Straalen, Nico M
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 14(2005), 7 vom: 03. Okt., Seite 727-39
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
|
| 773 |
1 |
8 |
|g volume:14
|g year:2005
|g number:7
|g day:03
|g month:10
|g pages:727-39
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_65
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 14
|j 2005
|e 7
|b 03
|c 10
|h 727-39
|