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20231227123534.0 |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n1221.xml
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|a (DE-627)NLM157244725
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|a (NLM)16105856
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Häkkinen, Suvi T
|e verfasserin
|4 aut
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|a Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6beta-hydroxylase
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 02.12.2005
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Hyoscyamine-6beta-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus muticus. The transformation was performed using a binary vector system based on Agrobacterium rhizogenes. Production of scopolamine in hairy roots was clearly correlated with the 35S-h6h transcript expression. The engineered N. tabacum and H. muticus hairy roots were studied for the production of scopolamine and other tropane and nicotine alkaloids after feeding the cultures with exogenous hyoscyamine. N. tabacum hairy roots carrying the 35S-h6h transgene showed a more efficient uptake of hyoscyamine from the culture medium and a higher rate of bioconversion of hyoscyamine to scopolamine than those of H. muticus. In particular, the secretion of scopolamine in N. tabacum hairy roots was remarkably high, up to 85% of the total scopolamine being released to the culture medium. Exogenous hyoscyamine also caused changes in nicotine alkaloid accumulation in N. tabacum hairy roots
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Alkaloids
|2 NLM
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|a Mixed Function Oxygenases
|2 NLM
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|a EC 1.-
|2 NLM
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|a hyoscyamine (6S)-dioxygenase
|2 NLM
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|a EC 1.14.11.11
|2 NLM
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|a Moyano, Elisabeth
|e verfasserin
|4 aut
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|a Cusidó, Rosa M
|e verfasserin
|4 aut
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|a Palazón, Javier
|e verfasserin
|4 aut
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|a Piñol, M Teresa
|e verfasserin
|4 aut
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|a Oksman-Caldentey, Kirsi-Marja
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 420 vom: 24. Okt., Seite 2611-8
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:56
|g year:2005
|g number:420
|g day:24
|g month:10
|g pages:2611-8
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|d 56
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|h 2611-8
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