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231223s2005 xx ||||| 00| ||eng c |
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|a (NLM)16089359
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lee, Jin Woo
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 03.04.2007
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|a Date Revised 21.11.2008
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|a published: Print
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|a Citation Status MEDLINE
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|a The development of proper biomaterials is critical for the success of cell therapy and modern tissue engineering. Here, we extruded the yolk and remaining inner mass from fertilized zebrafish eggs and used the resulting chorions as a biomaterial for the differentiation and attachment of mouse P19 embryonic carcinoma (EC) cells. Cells inserted into the chorion showed the spontaneous formation of embryoid body due to the repulsive cell adhesion of the chorion and differentiated specifically into neural cells and cardiomyocytes. In contrast, dissolved chorion extracellular matrix (ECM) conferred enhanced cell attachment on it, suggesting that a unique property of the zebrafish chorion with nanoporous structure appears to be responsible for the simple and controllable embryoid formation for stem cell differentiation. These results indicate that chorions from fertilized zebrafish eggs may be used as an extracellular matrix alternative and applied for stem cell differentiation to specific cell lineages
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Biocompatible Materials
|2 NLM
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| 700 |
1 |
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|a Na, Dae Seok
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chae, Su Kyoung
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Choong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Ji Yoon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ju, Byeong Kwon
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Hwang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kim, Sun Uk
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hwang, Chang Nam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Sang Ho
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 21(2005), 17 vom: 16. Aug., Seite 7615-20
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:21
|g year:2005
|g number:17
|g day:16
|g month:08
|g pages:7615-20
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