Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine
The aroA gene of Yersinia ruckeri, which encodes 5-enolpyruvylshikimate 3-phosphate synthase, was insertionally inactivated with a DNA fragment containing a kanamycin resistance determinant and reintroduced by allelic exchange into the chromosome of Y. ruckeri 21102 O1 by means of the suicide vector...
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 28(2005), 7 vom: 15. Juli, Seite 419-27 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Bacterial Vaccines |
| Zusammenfassung: | The aroA gene of Yersinia ruckeri, which encodes 5-enolpyruvylshikimate 3-phosphate synthase, was insertionally inactivated with a DNA fragment containing a kanamycin resistance determinant and reintroduced by allelic exchange into the chromosome of Y. ruckeri 21102 O1 by means of the suicide vector pIVET8. The Y. ruckeri aroA::Kan(r) mutant was highly attenuated when inoculated intraperitoneally into rainbow trout, with a 50% lethal dose of >5 x 10(7) CFU. The mutants were not recoverable from the internal organs 48 h post-inoculation or later. The vaccination of rainbow trout with the AroA mutant as a live vaccine conferred significant protection (relative percentage survival = 90%) against the pathogenic wild-type strain of Y. ruckeri |
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| Beschreibung: | Date Completed 21.11.2005 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1365-2761 |