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|a pubmed24n0523.xml
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|a (DE-627)NLM156846748
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|a (NLM)16049946
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Sandvoss, Martin
|e verfasserin
|4 aut
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|a HPLC-SPE-NMR in pharmaceutical development
|b capabilities and applications
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 23.09.2005
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|a Date Revised 15.11.2012
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Copyright 2005 John Wiley & Sons, Ltd.
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|a High-performance liquid chromatography-solid phase extraction-NMR spectroscopy (HPLC-SPE-NMR) has recently become commercially available and has been evaluated with regard to its applicability in a pharmaceutical environment. The addition of an automated SPE unit to an HPLC-NMR system for peak trapping results in an improved NMR signal-to-noise ratio (S/N) and also has other practical advantages. The trapping efficiency is shown to depend on compound polarity and is highest for compounds eluting late on reversed-phase HPLC systems. Multiple peak trapping further increases the S/N, again with the best results for less polar compounds. For polar compounds, multiple peak trapping resulted in no S/N gain as the amount of material retained on the SPE cartridge was equivalent to that from a single injection. When compared with conventional HPLC-NMR, a S/N gain of up to five-fold could be achieved for some compounds in a single trapping step. A major advantage of the technique is the independence of the chromatographic step from the NMR step, resulting in greater versatility than conventional HPLC-NMR in the HPLC solvents and NMR solvents that can be used. Practical applications from both drug metabolite and drug impurity identification are presented
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|a Journal Article
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|a Acetonitriles
|2 NLM
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|a Acids, Carbocyclic
|2 NLM
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|a Ketones
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|a Pharmaceutical Preparations
|2 NLM
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|a Solvents
|2 NLM
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|a acetonitrile
|2 NLM
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|a Z072SB282N
|2 NLM
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|a Bardsley, Ben
|e verfasserin
|4 aut
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|a Beck, Tony L
|e verfasserin
|4 aut
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|a Lee-Smith, Emma
|e verfasserin
|4 aut
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|a North, Stephanie E
|e verfasserin
|4 aut
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|a Moore, Peter J
|e verfasserin
|4 aut
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|a Edwards, Andrew J
|e verfasserin
|4 aut
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| 700 |
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|a Smith, Richard J
|e verfasserin
|4 aut
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|i Enthalten in
|t Magnetic resonance in chemistry : MRC
|d 1985
|g 43(2005), 9 vom: 01. Sept., Seite 762-70
|w (DE-627)NLM098179667
|x 1097-458X
|7 nnns
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| 773 |
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|g volume:43
|g year:2005
|g number:9
|g day:01
|g month:09
|g pages:762-70
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|d 43
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|e 9
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