GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana

Lateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. In Arabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. In order to study the mechanisms of lateral root development, a popul...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 56(2005), 419 vom: 16. Sept., Seite 2433-42
1. Verfasser: Laplaze, Laurent (VerfasserIn)
Weitere Verfasser: Parizot, Boris, Baker, Andrew, Ricaud, Lilian, Martinière, Alexandre, Auguy, Florence, Franche, Claudine, Nussaume, Laurent, Bogusz, Didier, Haseloff, Jim
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA-Binding Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Green Fluorescent Proteins 147336-22-9
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500 |a Citation Status MEDLINE 
520 |a Lateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. In Arabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. In order to study the mechanisms of lateral root development, a population of Arabidopsis GAL4-GFP enhancer trap lines were screened and two lines were isolated with GAL4 expression in root xylem-pole pericycle cells (J0121), i.e. in cells competent to become lateral root founder cells, and in young lateral root primordia (J0192). These two enhancer trap lines are very useful tools with which to study the molecular and cellular bases of lateral root development using targeted gene expression. These lines were used for genetic ablation experiments by targeting the expression of a toxin-encoding gene. Moreover, the molecular bases of the enhancer trap expression pattern were characterized. These results suggest that the lateral-root-specific GAL4 expression pattern in J0192 is due to a strong enhancer in the promoter of the LOB-domain protein gene LBD16 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a DNA Primers  |2 NLM 
650 7 |a DNA-Binding Proteins  |2 NLM 
650 7 |a GAL4 protein, S cerevisiae  |2 NLM 
650 7 |a Recombinant Fusion Proteins  |2 NLM 
650 7 |a Saccharomyces cerevisiae Proteins  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Green Fluorescent Proteins  |2 NLM 
650 7 |a 147336-22-9  |2 NLM 
700 1 |a Parizot, Boris  |e verfasserin  |4 aut 
700 1 |a Baker, Andrew  |e verfasserin  |4 aut 
700 1 |a Ricaud, Lilian  |e verfasserin  |4 aut 
700 1 |a Martinière, Alexandre  |e verfasserin  |4 aut 
700 1 |a Auguy, Florence  |e verfasserin  |4 aut 
700 1 |a Franche, Claudine  |e verfasserin  |4 aut 
700 1 |a Nussaume, Laurent  |e verfasserin  |4 aut 
700 1 |a Bogusz, Didier  |e verfasserin  |4 aut 
700 1 |a Haseloff, Jim  |e verfasserin  |4 aut 
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