Immobilization of histidine-tagged recombinant proteins onto micropatterned surfaces for cell-based functional assays
This letter describes a method for preparing protein microarrays that allow the functional analysis of proteins at a cellular level. This method involves the utilization of recombinant proteins genetically engineered to carry a fusion tag that has an affinity for metal ions. A micropatterned alkanet...
Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 21(2005), 16 vom: 02. Aug., Seite 7071-5 |
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Auteur principal: | |
Autres auteurs: | , |
Format: | Article |
Langue: | English |
Publié: |
2005
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Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
Sujets: | Journal Article Research Support, Non-U.S. Gov't Chelating Agents His-His-His-His-His-His Oligopeptides Recombinant Fusion Proteins Histidine 4QD397987E Epidermal Growth Factor 62229-50-9 |