Immobilization of histidine-tagged recombinant proteins onto micropatterned surfaces for cell-based functional assays

This letter describes a method for preparing protein microarrays that allow the functional analysis of proteins at a cellular level. This method involves the utilization of recombinant proteins genetically engineered to carry a fusion tag that has an affinity for metal ions. A micropatterned alkanet...

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Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 21(2005), 16 vom: 02. Aug., Seite 7071-5
Auteur principal: Kato, Koichi (Auteur)
Autres auteurs: Sato, Hideki, Iwata, Hiroo
Format: Article
Langue:English
Publié: 2005
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, Non-U.S. Gov't Chelating Agents His-His-His-His-His-His Oligopeptides Recombinant Fusion Proteins Histidine 4QD397987E Epidermal Growth Factor 62229-50-9