Molecular cloning and characterization of an almond 9-hydroperoxide lyase, a new CYP74 targeted to lipid bodies

Oxylipin metabolism represents one of many defence mechanisms employed by plants. It begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases to form fatty acid hydroperoxides that are substrates for several enzymes, including specialized cytochrome P450s known as CYP74s. The targ...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 56(2005), 419 vom: 15. Sept., Seite 2321-33
1. Verfasser: Mita, Giovanni (VerfasserIn)
Weitere Verfasser: Quarta, Angela, Fasano, Pasqua, De Paolis, Angelo, Di Sansebastiano, Gian Pietro, Perrotta, Carla, Iannacone, Rina, Belfield, Eric, Hughes, Richard, Tsesmetzis, Nicolas, Casey, Rod, Santino, Angelo
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers Recombinant Fusion Proteins Recombinant Proteins 9-hydroperoxide lyase EC 4.2.1.- Hydro-Lyases
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245 1 0 |a Molecular cloning and characterization of an almond 9-hydroperoxide lyase, a new CYP74 targeted to lipid bodies 
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500 |a Citation Status MEDLINE 
520 |a Oxylipin metabolism represents one of many defence mechanisms employed by plants. It begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases to form fatty acid hydroperoxides that are substrates for several enzymes, including specialized cytochrome P450s known as CYP74s. The targeting of a new CYP74, a 9-hydroperoxide lyase (HPL) from almonds, to the endomembrane system and lipid bodies, both as enzyme activity in almond seeds and as GFP fusions transiently expressed in tobacco protoplasts, is described. Such association of a CYP74 with lipid bodies has not been reported previously. Also described are the properties of a 9-HPL gene, the developmental regulation of its expression, the production and characterization of recombinant 9-HPL in Escherichia coli, and the developmental correlation between gene expression, enzyme activity, and the appearance of volatile C9 aldehydes from HPL action 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Recombinant Fusion Proteins  |2 NLM 
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650 7 |a 9-hydroperoxide lyase  |2 NLM 
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700 1 |a Quarta, Angela  |e verfasserin  |4 aut 
700 1 |a Fasano, Pasqua  |e verfasserin  |4 aut 
700 1 |a De Paolis, Angelo  |e verfasserin  |4 aut 
700 1 |a Di Sansebastiano, Gian Pietro  |e verfasserin  |4 aut 
700 1 |a Perrotta, Carla  |e verfasserin  |4 aut 
700 1 |a Iannacone, Rina  |e verfasserin  |4 aut 
700 1 |a Belfield, Eric  |e verfasserin  |4 aut 
700 1 |a Hughes, Richard  |e verfasserin  |4 aut 
700 1 |a Tsesmetzis, Nicolas  |e verfasserin  |4 aut 
700 1 |a Casey, Rod  |e verfasserin  |4 aut 
700 1 |a Santino, Angelo  |e verfasserin  |4 aut 
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