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01000caa a22002652 4500 |
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NLM156423480 |
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DE-627 |
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20250206125604.0 |
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231223s2005 xx ||||| 00| ||chi c |
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|a pubmed25n0522.xml
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|a (DE-627)NLM156423480
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|a (NLM)16004788
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Tang, Xiao-qin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Effect of naloxone on expression of Bcl-2 protein and tumor necrosis factor-alpha in rats with acute myocardial ischemia/reperfusion injury
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 05.01.2010
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To investigate the effect of naloxone on myocardial cell apoptosis and apoptosis-related gene Bcl-2 in rats with acute myocardial ischemia/reperfusion (AMIR) injury, and explore the mechanism of protective effect of naloxone on myocardium
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|a METHODS: Thirty SD rats were randomly divided into three groups (n=10): ischemia/reperfusion group, naloxone preconditioning group (naloxone was injected intraperitoneally 10 minutes before ischemia and 2 hours after reperfusion), and normal control group. The left anterior descending branch (LAD) of rat coronary artery was tied and un-tied in ischemia/reperfusion group and naloxone preconditioning group to establish the AMIR model in rats. The animals were then sacrificed and hearts were harvested. The expression of Bcl-2 protein was observed by immunohistochemical technique. Radioimmunoassay (RIA) was used to determine tumor necrosis factor-alpha (TNF-alpha) in serum
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|a RESULTS: In the normal control group, there was no Bcl-2 expression and TNF-alpha level was (0.39+/-0.06) mug/L. Higher expression of Bcl-2 and increased TNF-alpha levels were found in ischemia/reperfusion group. The expression of Bcl-2 protein increased significantly [(+++) vs. (+)], and TNF-alpha was significantly lower in naloxone preconditioning group than those in the normal control group [(0.55+/-0.12) microg/L vs. (0.86+/-0.11) microg/L, P<0.01]
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|a CONCLUSION: Naloxone can protect myocardium from AMIR injury by inhibiting the apoptosis of cardiomyocytes induced by TNF-alpha and up-regulating protein expression of bcl-2 gene
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|a English Abstract
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Proto-Oncogene Proteins c-bcl-2
|2 NLM
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| 650 |
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7 |
|a Tumor Necrosis Factor-alpha
|2 NLM
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| 650 |
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7 |
|a Naloxone
|2 NLM
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| 650 |
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7 |
|a 36B82AMQ7N
|2 NLM
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| 700 |
1 |
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|a Zhao, Jian-hong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Zheng-yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, Shao-li
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 17(2005), 7 vom: 11. Juli, Seite 430-2
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:17
|g year:2005
|g number:7
|g day:11
|g month:07
|g pages:430-2
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 17
|j 2005
|e 7
|b 11
|c 07
|h 430-2
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