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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed25n0521.xml
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|a (DE-627)NLM156349310
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|a (NLM)15996985
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a van Raemdonck, Damien
|e verfasserin
|4 aut
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| 245 |
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|a Molecular changes associated with the setting up of secondary growth in aspen
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 23.09.2005
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|a Date Revised 15.11.2006
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|a published: Print-Electronic
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|a GENBANK: AY780430, AY780431
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|a Citation Status MEDLINE
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|a Vascular secondary growth results from the activity of the vascular cambium, which produces secondary phloem and secondary xylem. By means of cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis along aspen stems, several potential regulatory genes involved in the progressive transition from primary to secondary growth were identified. A total of 83 unique transcript-derived fragments (TDFs) was found to be differentiated between the top and the bottom of the stem. An independent RT-PCR expression analysis validated the cDNA-AFLP profiles for 19 of the TDFs. Among these, seven correspond to new genes encoding putative regulatory proteins. Emphasis was laid upon two genes encoding, respectively, an AP2/ERF-like transcription factor (PtaERF1) and a RING finger protein (PtaRHE1); their differential expression was further confirmed by reverse northern analysis. In situ RT-PCR revealed that PtaERF1 was expressed in phloem tissue and that PtaRHE1 had a pronounced expression in ray initials and their derivatives within the cambial zone. These results suggest that these genes have a potential role in vascular tissue development and/or functioning
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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|a DNA, Plant
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Pesquet, Edouard
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cloquet, Sophie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Beeckman, Hans
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Boerjan, Wout
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Goffner, Deborah
|e verfasserin
|4 aut
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| 700 |
1 |
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|a El Jaziri, Mondher
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baucher, Marie
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 418 vom: 06. Aug., Seite 2211-27
|w (DE-627)NLM098182706
|x 0022-0957
|7 nnns
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| 773 |
1 |
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|g volume:56
|g year:2005
|g number:418
|g day:06
|g month:08
|g pages:2211-27
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|a AR
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|d 56
|j 2005
|e 418
|b 06
|c 08
|h 2211-27
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