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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0521.xml
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|a (DE-627)NLM15622898X
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|a (NLM)15984252
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Sostarecz, Audra G
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Influence of molecular environment on the analysis of phospholipids by time-of-flight secondary ion mass spectrometry
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| 264 |
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1 |
|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 22.05.2006
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|a Date Revised 26.10.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a Understanding the influence of molecular environment on phospholipids is important in time-of-flight secondary ion mass spectrometry (TOF-SIMS) studies of complex systems such as cellular membranes. Varying the molecular environment of model membrane Langmuir-Blodgett (LB) films is shown to affect the TOF-SIMS signal of the phospholipids in the films. The molecular environment of a LB film of dipalmitoylphosphatidylcholine (DPPC) is changed by varying the film density, varying the sample substrate, and the addition of cholesterol. An increase in film density results in a decrease in the headgroup fragment ion signal at a mass-to-charge ratio of 184 (phosphocholine). Varying the sample substrate increases the secondary ion yield of phosphocholine as does the addition of proton-donating molecules such as cholesterol to the DPPC LB film. Switching from a model system of DPPC and cholesterol to one of dipalmitoylphosphatidylethanolamine (DPPE) and cholesterol demonstrates the ability of cholesterol to also mask the phospholipid headgroup ion signal. TOF-SIMS studies of simplistic phospholipid LB model membrane systems demonstrate the potential use of these systems in TOF-SIMS analysis of cells
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, N.I.H., Extramural
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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7 |
|a Membranes, Artificial
|2 NLM
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| 650 |
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7 |
|a Phosphatidylethanolamines
|2 NLM
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| 650 |
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|a Phosphorylcholine
|2 NLM
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| 650 |
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7 |
|a 107-73-3
|2 NLM
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| 650 |
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7 |
|a 1,2-dipalmitoyl-3-phosphatidylethanolamine
|2 NLM
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| 650 |
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7 |
|a 3026-45-7
|2 NLM
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| 650 |
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|a Cholesterol
|2 NLM
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| 650 |
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7 |
|a 97C5T2UQ7J
|2 NLM
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| 700 |
1 |
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|a Cannon, Donald M
|c Jr
|e verfasserin
|4 aut
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| 700 |
1 |
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|a McQuaw, Carolyn M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Shixin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ewing, Andrew G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Winograd, Nicholas
|e verfasserin
|4 aut
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| 773 |
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8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 12 vom: 08. Juni, Seite 4926-32
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:20
|g year:2004
|g number:12
|g day:08
|g month:06
|g pages:4926-32
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|a GBV_ILN_721
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|a AR
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| 952 |
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|d 20
|j 2004
|e 12
|b 08
|c 06
|h 4926-32
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