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01000naa a22002652 4500 |
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NLM156217627 |
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DE-627 |
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20231223073720.0 |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0521.xml
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|a (DE-627)NLM156217627
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|a (NLM)15983009
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Li, Jian-Feng
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Tyr152 plays a central role in the catalysis of 1-aminocyclopropane-1-carboxylate synthase
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 23.09.2005
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a 1-Aminocyclopropane-1-carboxylate (ACC) synthase is a key enzyme in the regulation of ethylene biosynthesis in higher plants. To investigate the catalytic significances of two conserved tyrosine residues, Tyr151 and Tyr152, of a tomato ACC synthase isozyme (LeACS2), five ACC synthase mutants (Y151F, Y151G, Y152F, Y152G, and Y151F/Y152F) were constructed and over-expressed in Escherichia coli. Subsequent kinetic analysis indicated that these point mutations in mutants Y152F, Y152G, and Y151F/Y152F, either reduced the catalytic efficiency more than 98% or fully inactivated ACC synthase, while Y151F and Y151G mutants reduced the enzymatic activities by 27% and 83%, respectively. It is therefore concluded that Tyr152, especially its hydroxyl group, plays an essential role in the catalysis of ACC synthase. Thus, a revised catalytic model is hereby proposed for functional ACC synthase
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Amino Acids, Cyclic
|2 NLM
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| 650 |
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|a 1-aminocyclopropane-1-carboxylic acid
|2 NLM
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| 650 |
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|a 3K9EJ633GL
|2 NLM
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| 650 |
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|a Tyrosine
|2 NLM
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| 650 |
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|a 42HK56048U
|2 NLM
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| 650 |
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|a S-Adenosylmethionine
|2 NLM
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| 650 |
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|a 7LP2MPO46S
|2 NLM
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| 650 |
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|a Lyases
|2 NLM
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| 650 |
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7 |
|a EC 4.-
|2 NLM
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| 650 |
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|a 1-aminocyclopropanecarboxylate synthase
|2 NLM
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| 650 |
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|a EC 4.4.1.14
|2 NLM
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| 700 |
1 |
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|a Qu, Liang-Hu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Ning
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 418 vom: 05. Aug., Seite 2203-10
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
8 |
|g volume:56
|g year:2005
|g number:418
|g day:05
|g month:08
|g pages:2203-10
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| 912 |
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 56
|j 2005
|e 418
|b 05
|c 08
|h 2203-10
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