The interaction of boronic acid-substituted viologens with pyranine : the effects of quencher charge on fluorescence quenching and glucose response
The fluorescence sensing of several monosaccharides using boronic acid-substituted viologen quenchers in combination with the fluorescent dye pyranine (HPTS) is reported. In this two-component sensing system, fluorescence quenching by the viologen is modulated by monosaccharides to provide a fluores...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 21(2005), 14 vom: 05. Juli, Seite 6540-7 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2005
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Arylsulfonates Boronic Acids Viologens pyranine I2W85YOX9L Glucose IY9XDZ35W2 |
| Résumé: | The fluorescence sensing of several monosaccharides using boronic acid-substituted viologen quenchers in combination with the fluorescent dye pyranine (HPTS) is reported. In this two-component sensing system, fluorescence quenching by the viologen is modulated by monosaccharides to provide a fluorescence signal. A series of viologen quenchers with different charges were prepared and tested for their ability both to quench the fluorescence of HPTS and to sense changes in glucose concentration in aqueous solution at pH 7.4. Both quenching efficiency and sugar sensing were found to be strongly dependent upon viologen charge. The molar ratio between HPTS and each of the viologen quenchers was varied in order to obtain an optimal ratio that provided a fairly linear fluorescence signal across a physiological glucose concentration range. Both the quenching and sugar sensing results are explained by electrostatic interaction between dye and quencher |
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| Description: | Date Completed 21.08.2006 Date Revised 24.11.2016 published: Print Citation Status MEDLINE |
| ISSN: | 0743-7463 |