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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0520.xml
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|a (DE-627)NLM15607723X
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|a (NLM)15968125
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Iwamoto, Hiroyuki
|e verfasserin
|4 aut
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|a X-ray microdiffraction and conventional diffraction from frozen-hydrated biological specimens
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 23.08.2005
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|a Date Revised 10.12.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A system for recording microdiffraction patterns from micrometer-sized quick-frozen hydrated biological specimens at the high-flux beamline BL40XU of SPring-8 is described. The optics consists of a pair of pinholes drilled into tantalum substratum, with a defining aperture of diameter 2 microm. The frozen specimens are placed in an in-vacuum cryochamber mounted on a three-axis goniometer, where the specimens are stably held at a liquid-nitrogen temperature ( approximately 74 K). A beam size of 1.5 microm (full width at half-maximum) is attained at the sample position. By using this system, diffraction patterns have been recorded from an isolated single myofibril (diameter approximately 3 microm) of an insect flight muscle in an area equivalent to a single sarcomere (length approximately 3 microm). The technique is potentially applicable to other micrometer-sized hydrated biological specimens, which are more susceptible to radiation damage than dry synthetic polymers or biopolymers. The quick-freezing of biological specimens has also been proven useful in reducing the specimen volume in the beam in conventional diffraction recordings
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|a Evaluation Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Validation Study
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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1 |
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|a Inoue, Katsuaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fujisawa, Tetsuro
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yagi, Naoto
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 12(2005), Pt 4 vom: 21. Juli, Seite 479-83
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
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|g volume:12
|g year:2005
|g number:Pt 4
|g day:21
|g month:07
|g pages:479-83
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_40
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|a GBV_ILN_350
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|a GBV_ILN_2005
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|a AR
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|d 12
|j 2005
|e Pt 4
|b 21
|c 07
|h 479-83
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