Identification and characterization of mutants capable of rapid seed germination at 10 degrees C from activation-tagged lines of Arabidopsis thaliana
Five cold temperature germinating (ctg) mutants, completing germination at 10 degrees C faster than wild type, have been recovered from activation-tagged populations of Arabidopsis thaliana. Three (ctg10-D, 41-D, and 144-D) were tagged and segregated 3:1 for BASTA resistance in the F2 when crossed w...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 56(2005), 418 vom: 09. Aug., Seite 2059-69 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Gibberellins Abscisic Acid 72S9A8J5GW gibberellic acid BU0A7MWB6L Glucose IY9XDZ35W2 |
| Zusammenfassung: | Five cold temperature germinating (ctg) mutants, completing germination at 10 degrees C faster than wild type, have been recovered from activation-tagged populations of Arabidopsis thaliana. Three (ctg10-D, 41-D, and 144-D) were tagged and segregated 3:1 for BASTA resistance in the F2 when crossed with wild type. None of the tagged ctg mutants was disturbed in sensitivity to abscisic acid or glucose but all were less sensitive to GA4+7 and osmoticum. The other two mutants (ctg156 and ctg225) were recessive, BASTA sensitive, and exhibited a transparent testa (tt) phenotype. They were more sensitive to abscisic acid, paclobutrazol, and GA4+7 than wild type but had similar sensitivity to osmoticum. Dimethylaminocinnamaldehyde staining of seeds from the two tt mutants, compared with stained seeds from the publicly available tt lines 1-10, suggested that ctg156 was a new allele of tt1, while ctg225 was similar to tt7-1. However, reciprocal crosses determined that ctg156 was not allelic to tt1 while ctg225 was a new allele of tt7. When the gene was sequenced from ctg225 it was missing 10 bp in the second exon, resulting in the incorporation of two spurious amino acids (G282E and D283A) followed by a stop. The screen successfully recovered mutants completing germination faster than wild type at 10 degrees C |
|---|---|
| Beschreibung: | Date Completed 23.09.2005 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1460-2431 |