Improved T-DNA vector for tagging plant promoters via high-throughput luciferase screening

Improved T-DNA vector for tagging plant promoters via high-throughput luciferase screening

Transferred DNA (T-DNA) tagging is a powerful tool for tagging and in planta characterization of plant genes on a genome-wide scale. An improved promoter tagging vector is described here, which contains the codon-optimized luciferase (luc+) reporter gene 31 bp from the right border of the T-DNA. Com...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 38(2005), 5 vom: 15. Mai, Seite 763-70
1. Verfasser: Remy, Serge (VerfasserIn)
Weitere Verfasser: Thiry, Els, Coemans, Bert, Windelinckx, Saskia, Swennen, Rony, Sági, László
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Journal Article Research Support, Non-U.S. Gov't DNA, Bacterial Recombinant Proteins T-DNA Luciferases, Firefly EC 1.13.12.7