Changes in the ascorbate system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress

The involvement of the ascorbate (AsA) system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress was studied. The treatment of 5-day-old pumpkin seedlings with 50 microM aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, whil...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 162(2005), 5 vom: 10. Mai, Seite 529-36
1. Verfasser: Dipierro, Nunzio (VerfasserIn)
Weitere Verfasser: Mondelli, Donato, Paciolla, Costantino, Brunetti, Gennaro, Dipierro, Silvio
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Alum Compounds Free Radical Scavengers aluminum sulfate 34S289N54E Hydrogen Peroxide BBX060AN9V Ascorbic Acid PQ6CK8PD0R
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520 |a The involvement of the ascorbate (AsA) system in the response of pumpkin (Cucurbita pepo L.) roots to aluminium stress was studied. The treatment of 5-day-old pumpkin seedlings with 50 microM aluminium sulphate resulted in approximately 60% inhibition of root growth within 48-60 h of treatment, while aluminium accumulated in the roots reaching a maximum within 48h. During the same period, the hydrogen peroxide content of the roots was strongly enhanced. The increased level of hydrogen peroxide was matched by both increased ascorbate peroxidase (APX) (EC 1.11.1.11) activity and ascorbate free radical reductase (AFRR) (EC 1.1.5.4) activity, while dehydroascorbate reductase (DHAR) (EC 1.8.5.1) and glutathione reductase (GR) (EC 1.6.4.2) did not change. The levels of AsA in the roots were also increased by the Al treatment. It was concluded that an oxidative burst is probably involved in the toxicity of Al in pumpkin roots and that plants react to the enhanced production of reactive oxygen species by expressing higher levels of scavenging systems such as the AsA-APX system 
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700 1 |a Dipierro, Silvio  |e verfasserin  |4 aut 
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