Effects of free proline accumulation in petunias under drought stress

Petunias (Petunia hybrida cv. 'Mitchell') accumulate free proline (Pro) under drought-stress conditions. It is therefore believed that Pro acts as an osmoprotectant in plants subjected to drought conditions. Petunia plants were transformed by Delta(1)-pyrroline-5-carboxylate synthetase gen...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 56(2005), 417 vom: 31. Juli, Seite 1975-81
1. Verfasser: Yamada, Mika (VerfasserIn)
Weitere Verfasser: Morishita, Hiromasa, Urano, Kaoru, Shiozaki, Noriko, Yamaguchi-Shinozaki, Kazuko, Shinozaki, Kazuo, Yoshiba, Yoshu
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Water 059QF0KO0R Sodium Chloride 451W47IQ8X Proline 9DLQ4CIU6V
LEADER 01000naa a22002652 4500
001 NLM155702246
003 DE-627
005 20231223072701.0
007 tu
008 231223s2005 xx ||||| 00| ||eng c
028 5 2 |a pubmed24n0519.xml 
035 |a (DE-627)NLM155702246 
035 |a (NLM)15928013 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Yamada, Mika  |e verfasserin  |4 aut 
245 1 0 |a Effects of free proline accumulation in petunias under drought stress 
264 1 |c 2005 
336 |a Text  |b txt  |2 rdacontent 
337 |a ohne Hilfsmittel zu benutzen  |b n  |2 rdamedia 
338 |a Band  |b nc  |2 rdacarrier 
500 |a Date Completed 16.08.2005 
500 |a Date Revised 10.03.2022 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Petunias (Petunia hybrida cv. 'Mitchell') accumulate free proline (Pro) under drought-stress conditions. It is therefore believed that Pro acts as an osmoprotectant in plants subjected to drought conditions. Petunia plants were transformed by Delta(1)-pyrroline-5-carboxylate synthetase genes (AtP5CS from Arabidopsis thaliana L. or OsP5CS from Oryza sativa L.). The transgenic plants accumulated Pro and their drought tolerance was tested. The Pro content amounted to 0.57-1.01% of the total amino acids in the transgenic plants, or 1.5-2.6 times that in wild-type plants grown under normal conditions. The transgenic plant lines tolerated 14 d of drought stress, which confirms that both P5CS transgenes had full functionality. Exogenous L-Pro treatment caused the plants to accumulate Pro; plants treated with 5 mM L-Pro accumulated up to 18 times more free Pro than untreated plants. Exogenous L-Pro restricted the growth of wild-type petunias more than that of Arabidopsis plants. The capacity for free Pro accumulation might depend on the plant species. The growth of petunia plants was influenced not only by the Pro concentration in the plants, but by the ratio of the Pro content to the total amino acids, because the growth of the transgenic petunia plants appeared normal 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Water  |2 NLM 
650 7 |a 059QF0KO0R  |2 NLM 
650 7 |a Sodium Chloride  |2 NLM 
650 7 |a 451W47IQ8X  |2 NLM 
650 7 |a Proline  |2 NLM 
650 7 |a 9DLQ4CIU6V  |2 NLM 
700 1 |a Morishita, Hiromasa  |e verfasserin  |4 aut 
700 1 |a Urano, Kaoru  |e verfasserin  |4 aut 
700 1 |a Shiozaki, Noriko  |e verfasserin  |4 aut 
700 1 |a Yamaguchi-Shinozaki, Kazuko  |e verfasserin  |4 aut 
700 1 |a Shinozaki, Kazuo  |e verfasserin  |4 aut 
700 1 |a Yoshiba, Yoshu  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g 56(2005), 417 vom: 31. Juli, Seite 1975-81  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnns 
773 1 8 |g volume:56  |g year:2005  |g number:417  |g day:31  |g month:07  |g pages:1975-81 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 56  |j 2005  |e 417  |b 31  |c 07  |h 1975-81