|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM155569589 |
| 003 |
DE-627 |
| 005 |
20231223072420.0 |
| 007 |
tu |
| 008 |
231223s2005 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0519.xml
|
| 035 |
|
|
|a (DE-627)NLM155569589
|
| 035 |
|
|
|a (NLM)15914014
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Riemenschneider, Anja
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Impact of elevated H(2)S on metabolite levels, activity of enzymes and expression of genes involved in cysteine metabolism
|
| 264 |
|
1 |
|c 2005
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 27.10.2005
|
| 500 |
|
|
|a Date Revised 08.04.2022
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a The effects of elevated atmospheric hydrogen sulfide (H(2)S) levels (0.25, 0.5, and 0.75 microl l(-1)) have been investigated in a short-term exposure experiment (3-48 h) on the model plant Arabidopsis thaliana (L.) Heynh. in comparison to untreated control plants. The most pronounced effects of H(2)S fumigation could be observed on the metabolite level: the contents of the thiols cysteine and glutathione were increased up to 20- and fourfold, respectively. A direct positive correlation of the thiol contents with the H(2)S concentrations applied was observed. To elucidate the molecular basis for the increased thiol levels, enzyme activities, messenger RNA and protein steady-state levels of cysteine-synthesizing and degrading pathways have been determined. The enzyme activities of O-acetyl-l-serine(thiol)lyase (OAS-TL) (EC 4.2.99.8) and l-cysteine desulfhydrase (EC 4.4.1.-) proteins were not significantly higher at elevated H(2)S levels in comparison to untreated control plants. 3-Mercaptopyruvate sulfurtransferase (EC 2.8.1.2) activity was slightly higher after the longest H(2)S exposure times. Elevated H(2)S levels of 0.25 and 0.5 microl l(-1) had promoting effects on both mRNA and protein levels of cysteine-synthesizing and degrading enzymes whereas the highest H(2)S concentrations caused lower levels of expression combined with mild symptoms of oxidative stress, as the consequence of its phytotoxicity. The differences in the expression of the three different OAS-TL isoforms (cytoplasmic, plastidic and mitochondrial) by H(2)S were very small. Increasing concentrations of H(2)S and longer exposure times to H(2)S let to a reduction in the pool of O-acetyl-l-serine, the second precursor of cysteine, and N-acetyl-l-serine in the leaves and shoots, indicating a substrate depletion in agreement with the increased thiol levels
|
| 650 |
|
4 |
|a Comparative Study
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Enzymes
|2 NLM
|
| 650 |
|
7 |
|a Serine
|2 NLM
|
| 650 |
|
7 |
|a 452VLY9402
|2 NLM
|
| 650 |
|
7 |
|a O-acetylhomoserine (thiol)-lyase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.5.1.47
|2 NLM
|
| 650 |
|
7 |
|a Sulfurtransferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.8.1.-
|2 NLM
|
| 650 |
|
7 |
|a 3-mercaptopyruvate sulphurtransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.8.1.2
|2 NLM
|
| 650 |
|
7 |
|a Carbon-Oxygen Lyases
|2 NLM
|
| 650 |
|
7 |
|a EC 4.2.-
|2 NLM
|
| 650 |
|
7 |
|a Cystathionine gamma-Lyase
|2 NLM
|
| 650 |
|
7 |
|a EC 4.4.1.1
|2 NLM
|
| 650 |
|
7 |
|a Cysteine
|2 NLM
|
| 650 |
|
7 |
|a K848JZ4886
|2 NLM
|
| 650 |
|
7 |
|a N-acetylserine
|2 NLM
|
| 650 |
|
7 |
|a W98518XGZ3
|2 NLM
|
| 650 |
|
7 |
|a Hydrogen Sulfide
|2 NLM
|
| 650 |
|
7 |
|a YY9FVM7NSN
|2 NLM
|
| 700 |
1 |
|
|a Nikiforova, Victoria
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Hoefgen, Rainer
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a De Kok, Luit J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Papenbrock, Jutta
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 43(2005), 5 vom: 01. Mai, Seite 473-83
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:43
|g year:2005
|g number:5
|g day:01
|g month:05
|g pages:473-83
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 43
|j 2005
|e 5
|b 01
|c 05
|h 473-83
|