An anther-specific cysteine-rich protein of tomato localized to the tapetum and microspores
The tapetum is a nutritive tissue of the stamen that is essential for normal microspore development. While numerous tapetal-specific genes have been identified, little information is available on the localization and function of the proteins produced by these genes. The tapetally produced protein 5B...
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 162(2005), 4 vom: 20. Apr., Seite 457-64 |
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| Format: | Aufsatz |
| Sprache: | English |
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2005
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Plant Proteins Cysteine K848JZ4886 |
| Zusammenfassung: | The tapetum is a nutritive tissue of the stamen that is essential for normal microspore development. While numerous tapetal-specific genes have been identified, little information is available on the localization and function of the proteins produced by these genes. The tapetally produced protein 5B-CRP is cysteine-rich, has a secretory signal sequence and lacks an endoplasmic reticulum retention sequence. The 5B-CRP mRNA is expressed specifically within the tapetum and accumulates from premeiosis to tetrad release. Antibodies generated against an Escherichia coli fusion protein only recognized 5B-CRP in the reduced state. The 5B-CRP was detected as a 6 kDa protein in extracts of stamens from microspore meiosis through anthesis and was also observed in extracts from dehisced pollen. In situ, 5B-CRP was localized in stamens to the tapetum and the developing microspores, from the tetrad through early free microspore stages. Based on similarity to proteins with known functions, 5B-CRP may inhibit proteasome activity within the stamen locule |
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| Beschreibung: | Date Completed 19.07.2005 Date Revised 07.12.2022 published: Print Citation Status MEDLINE |
| ISSN: | 0176-1617 |