Minimizing DNA contamination by using UNG-coupled quantitative real-time PCR on degraded DNA samples : application to ancient DNA studies
PCR analyses of ancient and degraded DNA suffer from their extreme sensitivity to contamination by modern DNA originating, in particular, from carryover contamination with previously amplified or cloned material. Any strategy for limiting carryover contamination would also have to be compatible with...
| Veröffentlicht in: | BioTechniques. - 1993. - 38(2005), 4 vom: 14. Apr., Seite 569-75 |
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| Format: | Aufsatz |
| Sprache: | English |
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2005
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Technical Report DNA Primers DNA 9007-49-2 DNA Glycosylases EC 3.2.2.- Uracil-DNA Glycosidase Pyrophosphatases EC 3.6.1.- |