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| LEADER |
01000caa a22002652 4500 |
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NLM154863262 |
| 003 |
DE-627 |
| 005 |
20250206083216.0 |
| 007 |
tu |
| 008 |
231223s2005 xx ||||| 00| ||chi c |
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5 |
2 |
|a pubmed25n0516.xml
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| 035 |
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|a (DE-627)NLM154863262
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| 035 |
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|a (NLM)15836820
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| 040 |
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Yang, Ming-shi
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Association of different alleles in interleukin-1 family genes with the coded cytokines production and outcome of sepsis
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| 264 |
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1 |
|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 15.12.2009
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| 500 |
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|a Date Revised 19.04.2005
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To investigate the association of alleles in interleukin-1(IL-1) family genes with IL-1alpha, IL-1beta, IL-1ra production as well as mRNA expression and the outcome of sepsis
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| 520 |
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|a METHODS: Sixty septic patients and equal number of healthy volunteers were enrolled in this study. Monocytes of healthy volunteers segregated by Ficoll were stimulated by lipopolysaccharide (LPS) in vitro. IL-1alpha, IL-1beta and IL-1ra mRNA expressions were semi-quantitatively analyzed with reverse transcription-polymerase chain reaction (RT-PCR). Cytokine production was determined with enzyme linked immunoadsorbent assay(ELISA). Alleles in IL-1A (intron 6, VNTR), IL-1B (-511, RLFP) and IL-1RN (intron 2, VNTR) were screened in all cases
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| 520 |
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|a RESULTS: The number of individuals carrying allele of IL-1RN2 was significantly greater in septic patients than that in healthy volunteers. Mortality in septic patients with IL-1RN2, compared with IL-1RN1, was markedly higher. Notably, IL-1ra production as well as mRNA expression of monocytes challenged with LPS was significantly higher in IL-1RN2 allele than in IL-1RN1 carriers. However, there was no difference in the coded gene expression between alleles of IL-1A1 and IL-1A2, also between IL-1B1 and IL-1B2
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| 520 |
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|a CONCLUSION: IL-1RN2 allele induces high IL-1ra expression and is closely with the outcome of patients in sepsis. It could be one of high risk genetic factors in septic patients
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| 650 |
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4 |
|a English Abstract
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a IL1RN protein, human
|2 NLM
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| 650 |
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7 |
|a Interleukin 1 Receptor Antagonist Protein
|2 NLM
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| 650 |
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7 |
|a Interleukin-1
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Ma, Peng-lin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, De-chang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 17(2005), 4 vom: 18. Apr., Seite 203-6
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:17
|g year:2005
|g number:4
|g day:18
|g month:04
|g pages:203-6
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 17
|j 2005
|e 4
|b 18
|c 04
|h 203-6
|