Gene expression of C2 subunit of proteasome in cardiac muscle in burned rats with sepsis
OBJECTIVE: To study the gene expression of C2 subunit of proteasome and proteolysis in cardiac muscle in burned animals with sepsis
| Veröffentlicht in: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. - 1998. - 17(2005), 4 vom: 18. Apr., Seite 200-2 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | Chinese |
| Veröffentlicht: |
2005
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| Zugriff auf das übergeordnete Werk: | Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Methylhistidines RNA, Messenger Proteasome Endopeptidase Complex EC 3.4.25.1 3-methylhistidine MEH8O8Y0H0 |
| Zusammenfassung: | OBJECTIVE: To study the gene expression of C2 subunit of proteasome and proteolysis in cardiac muscle in burned animals with sepsis METHODS: Male Wistar rats were randomly divided into burn group, burn sepsis group and control group. The extent of burn injury was 30% total body surface area (TBSA) III degree burn, and the model of burn sepsis was replicated by administration of endotoxin (6 mg/kg) into the peritoneal cavity immediately after burn injury. The contents of 3-methylhistidine (3-MH) in cardiac muscle were determined with high performance liquid chromatography. The expression of C2 subunit mRNA in the cardiac muscle was assessed with Northern blot analysis RESULTS: The contents of 3-MH in cardiac muscle of burned rats with sepsis increased significantly at 2 hours and 6 hours, as compared with burned rats and normal control (all P<0.01). There was no marked difference of contents of 3-MH in cardiac muscle between burned rats at 2 hours and control group, however, the contents of 3-MH in cardiac muscle of burned rats at 6 hours increased significantly compared with control group. The gene expression of C2 subunit in cardiac muscle of burned rats with sepsis increased significantly at 2 hours and 6 hours compared with burned rats and normal control (all P<0.01). There were significant differences in the intensity of gene expression of C2 subunit in cardiac muscle between burned rats at 2 hours and 6 hours and normal control (all P<0.01) CONCLUSION: The data suggest that the activity of ubiquitin system, one of important protein degradation pathways, and the proteolysis in cardiac muscle is continuously enhanced after burn injury, especially when sepsis set in, probably contributes to abnormal protein metabolism with malfunction of the heart during burn sepsis |
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| Beschreibung: | Date Completed 15.12.2009 Date Revised 24.09.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1003-0603 |