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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0516.xml
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|a (DE-627)NLM154855103
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|a (NLM)15835974
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Hou, Yanxia
|e verfasserin
|4 aut
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| 245 |
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|a Study of Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile for odorant biosensors
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 28.07.2006
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|a Date Revised 15.11.2012
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|a published: Print
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|a Citation Status MEDLINE
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|a To make ultrathin films for the fabrication of artificial olfactory systems, odorant biosensors, we have investigated mixed Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile. Under optimized experimental conditions (phosphate buffer solution, pH 7.5, OBP-1F concentration of 4 mg L(-1), target pressure 35 mN m(-1)), the mixed monolayer at the air/water interface is very stable and has been efficiently transferred onto gold supports, which were previously functionalized by self-assembled monolayers (SAMs) with 1-octadecanethiol (ODT). Atomic force microscopy and electrochemical impedance spectroscopy were used to characterize mixed Langmuir-Blodgett (LB) films before and after contact with a specific odorant molecule, isoamyl acetate. AFM phase images show a higher contrast after contact with the odorant molecule due to the new structure of the OBP-1F/ODA LB film. Non-Faradaic electrochemical spectroscopy (EIS) is used to quantify the effect of the odorant based on the electrical properties of the OBP-1F/ODA LB film, as its resistance strongly decreases from 1.18 MOmega (before contact) to 25 kOmega (after contact)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Pentanols
|2 NLM
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|a Receptors, Odorant
|2 NLM
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|a Sulfhydryl Compounds
|2 NLM
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|a odorant-binding protein
|2 NLM
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|a n-octadecyl mercaptan
|2 NLM
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|a 2885-00-9
|2 NLM
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|a Gold
|2 NLM
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|a 7440-57-5
|2 NLM
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|a isoamyl acetate
|2 NLM
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|a Z135787824
|2 NLM
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1 |
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|a Jaffrezic-Renault, Nicole
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martelet, Claude
|e verfasserin
|4 aut
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|a Tlili, Chaker
|e verfasserin
|4 aut
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|a Zhang, Aidong
|e verfasserin
|4 aut
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|a Pernollet, Jean-Claude
|e verfasserin
|4 aut
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|a Briand, Loïc
|e verfasserin
|4 aut
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|a Gomila, Gabriel
|e verfasserin
|4 aut
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|a Errachid, Abdelhamid
|e verfasserin
|4 aut
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|a Samitier, Josep
|e verfasserin
|4 aut
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|a Salvagnac, Ludovic
|e verfasserin
|4 aut
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|a Torbiéro, Benoit
|e verfasserin
|4 aut
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|a Temple-Boyer, Pierre
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 21(2005), 9 vom: 26. Apr., Seite 4058-65
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:21
|g year:2005
|g number:9
|g day:26
|g month:04
|g pages:4058-65
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|d 21
|j 2005
|e 9
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|c 04
|h 4058-65
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