Surface enzyme kinetics for biopolymer microarrays : a combination of Langmuir and Michaelis-Menten concepts

Real-time surface plasmon resonance (SPR) imaging measurements of surface enzymatic reactions on DNA microarrays are analyzed using a kinetics model that couples the contributions of both enzyme adsorption and surface enzyme reaction kinetics. For the case of a 1:1 binding of an enzyme molecule (E)...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 21(2005), 9 vom: 26. Apr., Seite 4050-7
1. Verfasser: Lee, Hye Jin (VerfasserIn)
Weitere Verfasser: Wark, Alastair W, Goodrich, Terry T, Fang, Shiping, Corn, Robert M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Enzymes Enzymes, Immobilized DNA 9007-49-2 Exodeoxyribonucleases mehr... EC 3.1.- exodeoxyribonuclease III EC 3.1.11.2