Surface enzyme kinetics for biopolymer microarrays : a combination of Langmuir and Michaelis-Menten concepts

Real-time surface plasmon resonance (SPR) imaging measurements of surface enzymatic reactions on DNA microarrays are analyzed using a kinetics model that couples the contributions of both enzyme adsorption and surface enzyme reaction kinetics. For the case of a 1:1 binding of an enzyme molecule (E)...

Description complète

Détails bibliographiques
Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 21(2005), 9 vom: 26. Apr., Seite 4050-7
Auteur principal: Lee, Hye Jin (Auteur)
Autres auteurs: Wark, Alastair W, Goodrich, Terry T, Fang, Shiping, Corn, Robert M
Format: Article
Langue:English
Publié: 2005
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Enzymes Enzymes, Immobilized DNA 9007-49-2 Exodeoxyribonucleases plus... EC 3.1.- exodeoxyribonuclease III EC 3.1.11.2