Fluorescent assays to quantitate enzymatic activities yielding as end product an aqueous-insoluble indigo-blue dye
Hydrolytic enzymes acting upon indoxyl-derivatized substrates produce a water-insoluble indigo-blue dye. The generation of indigo dye works well in nonquantitative histochemical or diagnostic assays. For quantitative analyses however, the technique is unsuited. In this paper two fluorescent methods...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 20(2004), 6 vom: 16. März, Seite 2424-8 |
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| Auteur principal: | |
| Format: | Article |
| Langue: | English |
| Publié: |
2004
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Coloring Agents Enzymes Fluorescent Dyes Water 059QF0KO0R Indigo Carmine D3741U8K7L Neuraminidase |