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01000naa a22002652 4500 |
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NLM154530573 |
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DE-627 |
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20231223070206.0 |
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tu |
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231223s2004 xx ||||| 00| ||eng c |
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|a pubmed24n0515.xml
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|a (DE-627)NLM154530573
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|a (NLM)15801458
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wang, Hua
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Probing the orientation of surface-immobilized immunoglobulin G by time-of-flight secondary ion mass spectrometry
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| 264 |
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1 |
|c 2004
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 29.12.2005
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|a Date Revised 26.10.2019
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Static time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful surface analysis technique for the characterization of protein films because of its chemical selectivity and surface sensitivity. In this study, static ToF-SIMS and principal component analysis (PCA), a multivariate data analysis method, were combined to probe the orientation of surface-immobilized immunoglobulin G (IgG). IgG orientation can enhance its ability to detect its antigen in immunoassay techniques. The IgG used in this work is the mouse monoclonal anti-human chorionic gonadotropin (anti-hCG). Anti-hCG films on different well-defined substrates have been studied using its F(ab')2 and Fc fragments as references. Atomic force microscopy was used to characterize these protein films before static ToF-SIMS analysis. The results from PCA of ToF-SIMS spectra were related to the antibody primary amino acid composition and its three-dimensional structure
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, N.I.H., Extramural
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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7 |
|a Antibodies, Monoclonal
|2 NLM
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| 650 |
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7 |
|a Gonadotropins
|2 NLM
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| 650 |
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7 |
|a Immunoglobulin Fab Fragments
|2 NLM
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| 650 |
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7 |
|a Immunoglobulin Fc Fragments
|2 NLM
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| 650 |
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7 |
|a Immunoglobulin G
|2 NLM
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| 700 |
1 |
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|a Castner, David G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ratner, Buddy D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Shaoyi
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 20(2004), 5 vom: 02. März, Seite 1877-87
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
8 |
|g volume:20
|g year:2004
|g number:5
|g day:02
|g month:03
|g pages:1877-87
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a AR
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| 952 |
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|d 20
|j 2004
|e 5
|b 02
|c 03
|h 1877-87
|