Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements

Rice plants (Oryza sativa L.) utilize the iron chelators known as mugineic acid family phytosiderophores (MAs) to acquire iron from the rhizosphere. Synthesis of MAs and uptake of MA-chelated iron are strongly induced under conditions of iron deficiency. Microarray analysis was used to characterize...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 56(2005), 415 vom: 15. Mai, Seite 1305-16
1. Verfasser: Kobayashi, Takanori (VerfasserIn)
Weitere Verfasser: Suzuki, Motofumi, Inoue, Haruhiko, Itai, Reiko Nakanishi, Takahashi, Michiko, Nakanishi, Hiromi, Mori, Satoshi, Nishizawa, Naoko K
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Enzymes Iron Chelating Agents Plant Proteins RNA, Messenger RNA, Plant S-Adenosylmethionine 7LP2MPO46S Methionine AE28F7PNPL mehr... Iron E1UOL152H7
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100 1 |a Kobayashi, Takanori  |e verfasserin  |4 aut 
245 1 0 |a Expression of iron-acquisition-related genes in iron-deficient rice is co-ordinately induced by partially conserved iron-deficiency-responsive elements 
264 1 |c 2005 
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500 |a Date Revised 10.04.2022 
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520 |a Rice plants (Oryza sativa L.) utilize the iron chelators known as mugineic acid family phytosiderophores (MAs) to acquire iron from the rhizosphere. Synthesis of MAs and uptake of MA-chelated iron are strongly induced under conditions of iron deficiency. Microarray analysis was used to characterize the expression profile of rice in response to iron deficiency at the genomic level. mRNA extracted from iron-deficient or iron-sufficient rice roots or leaves was hybridized to a rice array containing 8987 cDNA clones. An induction ratio of greater than 2.0 in roots was observed for 57 genes, many of which are involved in iron-uptake mechanisms, including every identified or predicted step in the methionine cycle and the biosynthesis of MAs from methionine. Northern analysis confirmed that the expression of genes encoding every step in the methionine cycle is thoroughly induced by iron deficiency in roots, and almost thoroughly induced in leaves. A promoter search revealed that the iron-deficiency-induced genes related to iron uptake possessed sequences homologous to the iron-deficiency-responsive cis-acting elements IDE1 and IDE2 in their promoter regions, at a higher rate than that showing no induction under Fe deficiency. These results suggest that rice genes involved in iron acquisition are co-ordinately regulated by conserved mechanisms in response to iron deficiency, in which IDE-mediated regulation plays a significant role 
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650 7 |a Methionine  |2 NLM 
650 7 |a AE28F7PNPL  |2 NLM 
650 7 |a Iron  |2 NLM 
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700 1 |a Suzuki, Motofumi  |e verfasserin  |4 aut 
700 1 |a Inoue, Haruhiko  |e verfasserin  |4 aut 
700 1 |a Itai, Reiko Nakanishi  |e verfasserin  |4 aut 
700 1 |a Takahashi, Michiko  |e verfasserin  |4 aut 
700 1 |a Nakanishi, Hiromi  |e verfasserin  |4 aut 
700 1 |a Mori, Satoshi  |e verfasserin  |4 aut 
700 1 |a Nishizawa, Naoko K  |e verfasserin  |4 aut 
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