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231223s2005 xx ||||| 00| ||eng c |
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|a (NLM)15779955
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Vacklin, Hanna P
|e verfasserin
|4 aut
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| 245 |
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|a Composition of supported model membranes determined by neutron reflection
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 14.06.2006
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a We have investigated the formation of supported bilayers by coadsorption of dipalmitoyl phosphatidylcholine (DPPC) with the nonionic surfactant beta-D-dodecyl maltoside. The adsorption of mixed phospholipid-surfactant micelles on hydrophilic silica surfaces at 25 degrees C was followed as a function of bulk concentration by neutron reflection. Using chain-deuterated d(25)-beta-D-dodecyl maltoside and d(62)-DPPC, we demonstrate that it is possible to determine the composition of the bilayers at each stage of a sequential dilution process, which enriches the adsorbed layer in phospholipid and leads to complete elimination of the surfactant. The final supported bilayers have thicknesses of 51 +/- 3 A and are stable to heating to 37 degrees C once all surfactant has been removed, and the structures agree well with other published data on DPPC supported bilayers. The coadsorption of cholesterol in a DPPC-surfactant mixture was also achieved, and the location and volume fraction of cholesterol in the DPPC bilayer was determined. Cholesterol is located in a 18 +/- 1 A thick layer below the lipid headgroup region and leads to an increased bilayer thickness of 58 +/- 2 A at 26 mol % of cholesterol
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Glucosides
|2 NLM
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| 650 |
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|a Lipid Bilayers
|2 NLM
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|a Phosphatidylcholines
|2 NLM
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|a dodecyl maltoside
|2 NLM
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|a 69227-93-6
|2 NLM
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|a Cholesterol
|2 NLM
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|a 97C5T2UQ7J
|2 NLM
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| 700 |
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|a Tiberg, Fredrik
|e verfasserin
|4 aut
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1 |
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|a Fragneto, Giovanna
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thomas, Robert K
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 21(2005), 7 vom: 29. März, Seite 2827-37
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:21
|g year:2005
|g number:7
|g day:29
|g month:03
|g pages:2827-37
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