Stable maintenance and expression of a foreign gene in transgenic pear shoots retrieved from in vitro conservation
Shoot-tips of transgenic pear were successfully conserved in vitro by slow-growth and cryopreservation methods. After 1 year of storage in slow growth conditions, all shoot-tips survived showing shoot re-growth. Similarly, shoot-tips showed high survival and regeneration rates after cryopreservation...
Publié dans: | Journal of plant physiology. - 1979. - 162(2005), 2 vom: 06. Feb., Seite 237-43 |
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Auteur principal: | |
Autres auteurs: | , |
Format: | Article |
Langue: | English |
Publié: |
2005
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Accès à la collection: | Journal of plant physiology |
Sujets: | Journal Article Research Support, Non-U.S. Gov't |
Résumé: | Shoot-tips of transgenic pear were successfully conserved in vitro by slow-growth and cryopreservation methods. After 1 year of storage in slow growth conditions, all shoot-tips survived showing shoot re-growth. Similarly, shoot-tips showed high survival and regeneration rates after cryopreservation. The genetic stability of the transgenic GUS construct in shoots recovered from slow-growth and cryopreservation was assessed. The results from polymerase chain reaction and Southern blotting showed the stable maintenance of the GUS gene in genome, and the Single-Strand Conformation Polymorphism assay did not detect single base variation. X-Gluc staining suggested a normal expression of the GUS gene in shoots retrieved from these storage methods |
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Description: | Date Completed 26.05.2005 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
ISSN: | 1618-1328 |