Stable maintenance and expression of a foreign gene in transgenic pear shoots retrieved from in vitro conservation

Shoot-tips of transgenic pear were successfully conserved in vitro by slow-growth and cryopreservation methods. After 1 year of storage in slow growth conditions, all shoot-tips survived showing shoot re-growth. Similarly, shoot-tips showed high survival and regeneration rates after cryopreservation...

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Détails bibliographiques
Publié dans:Journal of plant physiology. - 1979. - 162(2005), 2 vom: 06. Feb., Seite 237-43
Auteur principal: Hao, Yu-Jin (Auteur)
Autres auteurs: Cheng, Yun-Jiang, Deng, Xiu-Xin
Format: Article
Langue:English
Publié: 2005
Accès à la collection:Journal of plant physiology
Sujets:Journal Article Research Support, Non-U.S. Gov't
Description
Résumé:Shoot-tips of transgenic pear were successfully conserved in vitro by slow-growth and cryopreservation methods. After 1 year of storage in slow growth conditions, all shoot-tips survived showing shoot re-growth. Similarly, shoot-tips showed high survival and regeneration rates after cryopreservation. The genetic stability of the transgenic GUS construct in shoots recovered from slow-growth and cryopreservation was assessed. The results from polymerase chain reaction and Southern blotting showed the stable maintenance of the GUS gene in genome, and the Single-Strand Conformation Polymorphism assay did not detect single base variation. X-Gluc staining suggested a normal expression of the GUS gene in shoots retrieved from these storage methods
Description:Date Completed 26.05.2005
Date Revised 30.09.2020
published: Print
Citation Status MEDLINE
ISSN:1618-1328