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20231223065502.0 |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0514.xml
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|a (DE-627)NLM154178381
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|a (NLM)15763667
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Mariano, André B
|e verfasserin
|4 aut
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| 245 |
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|a Sensitivities of the alternative respiratory components of potato tuber mitochondria to thiol reagents and Ca2+
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 04.05.2005
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Plant mitochondria differ from those of mammals, since they incorporate an alternative electron transport pathway, which branches at ubiquinol to an alternative oxidase (AOX), characteristically inhibited by salicylhydroxamic acid (SHAM). Another feature of plant mitochondria is that besides complex I (EC 1.6.5.3) they possess alternative NAD(P)H-dehydrogenases insensitive to rotenone. Many stress conditions are known to alter the expression of the alternative electron transport pathway in plant mitochondria. In the present study we investigated the effects of some thiol reagents and Ca(2+) on potato mitochondrial respiratory chain presenting different activities of the alternative respiratory components AOX and external NADH dehydrogenase, a condition induced by previous treatment of potato tubers (Solanum tuberosum L., cv. Bintje) to cold stress. The results showed that Ca(2+) presented an inhibitory effect on AOX pathway in potato mitochondria energized with NADH or succinate, which was only now observed when the cytochrome pathway was inhibited by cyanide. When the cytochrome pathway was functional, Ca(2+) stimulated the external NADH dehydrogenase. Diamide was a potent AOX inhibitor and this effect was only now observed when the cytochrome pathway was inactive, as was the case for the calcium ion. Mersalyl inhibited the externally located NADH dehydrogenase and had no effect on AOX activity. The results may represent an important function of Ca(2+) on the alternative mitochondrial enzymes NADH-DH(ext) and AOX
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Mitochondrial Proteins
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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|a Sulfhydryl Reagents
|2 NLM
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| 650 |
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7 |
|a Diamide
|2 NLM
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| 650 |
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7 |
|a 10465-78-8
|2 NLM
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| 650 |
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7 |
|a Ubiquinone
|2 NLM
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| 650 |
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7 |
|a 1339-63-5
|2 NLM
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| 650 |
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7 |
|a Mersalyl
|2 NLM
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| 650 |
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7 |
|a 5X1IO031V8
|2 NLM
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| 650 |
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|a Succinic Acid
|2 NLM
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| 650 |
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7 |
|a AB6MNQ6J6L
|2 NLM
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| 650 |
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|a Oxidoreductases
|2 NLM
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| 650 |
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|a EC 1.-
|2 NLM
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| 650 |
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7 |
|a alternative oxidase
|2 NLM
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| 650 |
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7 |
|a EC 1.-
|2 NLM
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| 650 |
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7 |
|a NADH Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.6.99.3
|2 NLM
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| 650 |
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7 |
|a ubiquinol
|2 NLM
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| 650 |
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7 |
|a M9NL0C577Y
|2 NLM
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| 650 |
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|a Oxygen
|2 NLM
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| 650 |
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|a S88TT14065
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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7 |
|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Valente, Caroline
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cadena, Silvia M S C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rocha, Maria E M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a de Oliveira, Maria B M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Carnieri, Eva G S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 43(2005), 1 vom: 28. Jan., Seite 61-7
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
8 |
|g volume:43
|g year:2005
|g number:1
|g day:28
|g month:01
|g pages:61-7
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 43
|j 2005
|e 1
|b 28
|c 01
|h 61-7
|