Community changes during start-up in methanogenic bioreactors exposed to increasing levels of ammonia
To investigate the methanogenic population dynamics during the start-up under gradually increasing free ammonia levels, five mesophilic UASB reactors seeded with different sludges were operated for 140 days. Changes in the methanogenic community investigated by using 16S rDNA/rRNA based molecular me...
| Veröffentlicht in: | Environmental technology. - 1998. - 26(2005), 1 vom: 31. Jan., Seite 85-91 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Zugriff auf das übergeordnete Werk: | Environmental technology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA, Bacterial RNA, Ribosomal, 16S Ammonia 7664-41-7 Methane OP0UW79H66 |
| Zusammenfassung: | To investigate the methanogenic population dynamics during the start-up under gradually increasing free ammonia levels, five mesophilic UASB reactors seeded with different sludges were operated for 140 days. Changes in the methanogenic community investigated by using 16S rDNA/rRNA based molecular methods such as denaturing gradient gel electrophoresis (DGGE), fluorescent in-situ hybridization (FISH), cloning and DNA sequencing. Free ammonia nitrogen (FAN) in the reactors was gradually increased from 50 to 130 mg l(-1) by increasing total ammonia nitrogen (TAN) from 1000 to 2500 mg l(-1) in the feed and keeping reactor pH at 7.7 in the active zone. Even at highest FAN level, COD and VFA removal efficiencies above 90 and 98% were obtained, respectively. However, Methanosaeta-related species mainly prevailing in seed sludges were substituted for Methanosarcina as the abundant methanogens although reactor performances were almost comparable and constant |
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| Beschreibung: | Date Completed 24.05.2005 Date Revised 31.03.2022 published: Print Citation Status MEDLINE |
| ISSN: | 0959-3330 |