Root cell patterning : a primary target for aluminium toxicity in maize

The short-term influence (5-180 min) of 50 microM Al on cell division was investigated in root tips of two Zea mays L. varieties differing in Al-resistance. The incorporation of bromodeoxyuridine into S-phase nuclei was visualized by immunofluorescence staining using confocal laser fluorescence micr...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 56(2005), 414 vom: 06. Apr., Seite 1213-20
1. Verfasser: Doncheva, Snezhanka (VerfasserIn)
Weitere Verfasser: Amenós, Montserrat, Poschenrieder, Charlotte, Barceló, Juan
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Aluminum CPD4NFA903
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520 |a The short-term influence (5-180 min) of 50 microM Al on cell division was investigated in root tips of two Zea mays L. varieties differing in Al-resistance. The incorporation of bromodeoxyuridine into S-phase nuclei was visualized by immunofluorescence staining using confocal laser fluorescence microscopy. In Al-sensitive plants 5 min Al exposure was enough to inhibit cell division in the proximal meristem (250-800 microm from the tip). After 10 or 30 min with Al only, a few S-phase nuclei were found in the cortical initials. By contrast, cell division was stimulated in the distal elongation zone (2.5-3.1 mm). After 180 min the protrusion of an incipient lateral root was observed in this zone. These observations suggest a fast change in cell patterning rather than a general cariotoxic effect after exposure to Al for a short time. No such changes were found in Al-resistant maize. This is the first report showing such fast Al-induced alterations in the number and the position of dividing cells in root tips. The observation that similar changes were induced by a local supply of naphthylphthalamic acid to the distal transition zone suggests that inhibition of auxin transport plays a role in the Al-induced alteration of root cell patterning 
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700 1 |a Barceló, Juan  |e verfasserin  |4 aut 
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