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20231223065015.0 |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0513.xml
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|a (DE-627)NLM153933453
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|a (NLM)15737980
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Bourgis, Fabienne
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamily
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 05.07.2005
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|a Date Revised 24.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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| 520 |
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|a An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Preliminary phylogenetic studies indicated that At-74 cDNA and its close homologue in Arabidopsis, At-74H, belong, however, to an equally distinct group. At-74 was ubiquitously expressed in vegetative organs and induced by glucose. The At-74 cDNA was overexpressed in A. thaliana to investigate its function, but this overexpression did not result in a clear phenotype. Enzymatic assays performed on At-74-overproducing transgenic plants or E. coli cells showed no increase in either the activities of cofactor-dependent and -independent phosphoglycerate mutases (i-PGMases) and F2,6Pase or that of acid phosphatases. The possible role of At-74 in plant metabolism was further investigated by carbon partitioning experiments with [U-(14)C] glucose and measurements of soluble sugars in both young leaves and roots. Two overexpressing At-74 lines showed a clear increase in glucose uptake. This paper introduces the At-74 homologue of the d-PGMase superfamily members and supports a possible role of At-74 in carbohydrate metabolism
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Carbon Radioisotopes
|2 NLM
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| 650 |
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|a DNA Primers
|2 NLM
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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|a DNA, Plant
|2 NLM
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| 650 |
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|a Phosphofructokinases
|2 NLM
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| 650 |
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7 |
|a EC 2.7.1 -
|2 NLM
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| 650 |
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|a Phosphoglycerate Mutase
|2 NLM
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| 650 |
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7 |
|a EC 5.4.2.11
|2 NLM
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| 700 |
1 |
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|a Botha, Fredrik C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mani, Srikrishnan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hiten, Fletcher N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rigden, Daniel J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Verbruggen, Nathalie
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 414 vom: 06. Apr., Seite 1129-42
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:56
|g year:2005
|g number:414
|g day:06
|g month:04
|g pages:1129-42
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 56
|j 2005
|e 414
|b 06
|c 04
|h 1129-42
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