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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed25n0513.xml
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|a (DE-627)NLM15380016X
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|a (NLM)15723824
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Khodakovskaya, Mariya
|e verfasserin
|4 aut
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|a Effects of cor15a-IPT gene expression on leaf senescence in transgenic Petunia x hybrida and Dendranthema x grandiflorum
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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500 |
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|a Date Completed 05.07.2005
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|a Date Revised 18.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a To prevent leaf senescence of young transplants or excised shoots during storage under dark and cold conditions, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of a cold-inducible promoter cor15a from Arabidopsis thaliana and introduced into Petunia x hybrida 'Marco Polo Odyssey' and Dendranthema x grandiflorum (chrysanthemum) 'Iridon'. Transgenic cor15a-ipt petunia and chrysanthemum plants and excised leaves remained green and healthy during prolonged dark storage (4 weeks at 25 degrees C) after an initial exposure to a brief cold-induction period (4 degrees C for 72 h). However, cor15a-ipt chrysanthemum plants and excised leaves that were not exposed to a cold-induction period, senesced under the same dark storage conditions. Regardless of cold-induction treatment, leaves and plants of non-transformed plants senesced under prolonged dark storage. Analysis of ipt expression indicated a marked increase in gene expression in intact transgenic plants as well as in isolated transgenic leaves exposed to a short cold-induction treatment prior to dark storage. These changes correlated with elevated concentrations of cytokinins in transgenic leaves after cold treatment. Cor15a-ipt transgenic plants showed a normal phenotype when grown at 25 degrees C
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA Primers
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Chlorophyll
|2 NLM
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|a 1406-65-1
|2 NLM
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|a Alkyl and Aryl Transferases
|2 NLM
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650 |
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7 |
|a EC 2.5.-
|2 NLM
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7 |
|a adenylate isopentenyltransferase
|2 NLM
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650 |
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7 |
|a EC 2.5.1.27
|2 NLM
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700 |
1 |
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|a Li, Yi
|e verfasserin
|4 aut
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700 |
1 |
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|a Li, Jisheng
|e verfasserin
|4 aut
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700 |
1 |
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|a Vanková, Radomíra
|e verfasserin
|4 aut
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1 |
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|a Malbeck, Jirí
|e verfasserin
|4 aut
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1 |
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|a McAvoy, Richard
|e verfasserin
|4 aut
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773 |
0 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 56(2005), 414 vom: 22. Apr., Seite 1165-75
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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773 |
1 |
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|g volume:56
|g year:2005
|g number:414
|g day:22
|g month:04
|g pages:1165-75
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|d 56
|j 2005
|e 414
|b 22
|c 04
|h 1165-75
|