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DE-627 |
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20231223062814.0 |
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231223s2005 xx ||||| 00| ||eng c |
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|a pubmed24n0510.xml
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|a (DE-627)NLM152824766
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|a (NLM)15620313
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Peelen, Dora
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Immobilization of amine-modified oligonucleotides on aldehyde-terminated alkanethiol monolayers on gold
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| 264 |
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|c 2005
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 28.06.2006
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|a Date Revised 14.11.2007
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|a published: Print
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|a Citation Status MEDLINE
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|a Chemistry is described for the fabrication of DNA arrays on gold surfaces. Alkanethiols modified with terminal aldehyde groups are used to prepare a self-assembled monolayer (SAM). The aldehyde groups of the monolayer may be reacted with amine-modified oligonucleotides or other amine-bearing biomolecules to form a Schiff base, which may then be reduced to a stable secondary amine by treatment with sodium cyanoborohydride. The surface modifications and reactions are characterized by polarization modulation Fourier transform infrared reflection absorption spectroscopy (PM-FTIRRAS), and the accessibility, binding specificity, and stability of the DNA-modified surfaces are demonstrated in hybridization experiments
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| 650 |
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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|a Aldehydes
|2 NLM
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| 650 |
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|a Amines
|2 NLM
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| 650 |
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7 |
|a Oligonucleotides
|2 NLM
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| 650 |
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|a Gold
|2 NLM
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| 650 |
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|a 7440-57-5
|2 NLM
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| 700 |
1 |
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|a Smith, Lloyd M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 21(2005), 1 vom: 04. Jan., Seite 266-71
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:21
|g year:2005
|g number:1
|g day:04
|g month:01
|g pages:266-71
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|a AR
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|d 21
|j 2005
|e 1
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|h 266-71
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