Stepwise synthesis of Gly-Gly-His on gold surfaces modified with mixed self-assembled monolayers
Peptide-modified electrode surfaces have been shown to have excellent recognition properties for metal ions. An efficient method of screening a potential peptide for its selectivity for a given metal would involve the synthesis of the peptide directly on the electrode surface. This paper outlines a...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 21(2005), 1 vom: 04. Jan., Seite 260-5 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Oligopeptides Histidine 4QD397987E Gold 7440-57-5 3-Mercaptopropionic Acid B03TJ3QU9M Glycine TE7660XO1C |
| Zusammenfassung: | Peptide-modified electrode surfaces have been shown to have excellent recognition properties for metal ions. An efficient method of screening a potential peptide for its selectivity for a given metal would involve the synthesis of the peptide directly on the electrode surface. This paper outlines a procedure in which the tripeptide Gly-Gly-His was synthesized one amino acid at a time on a gold surface modified with a self-assembled monolayer of the mixed alkanethiolates 3-mercaptopropionic acid (MPA) and 3-mercaptopropane (MP). Electrochemistry and high-resolution mass spectrometry were used to elucidate the structure of the adsorbed species and follow the synthesis. The amino acids can be attached only to MPA, but the presence of a diluting unreactive molecule of MP reduces steric crowding about the reaction center. The maximum coverage of synthesized tripeptide occurs at a ratio of MPA/MP of 1:1 |
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| Beschreibung: | Date Completed 28.06.2006 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 0743-7463 |