A mini exon in the sucrose:sucrose 1-fructosyltransferase gene of wheat

We previously reported the cloning of a wheat sucrose:sucrose 1-fructosyltransferase (1-SST) cDNA, designated wft2. Wft2 proteins have fructosyltransferase enzyme activity and initiate fructan synthesis (Biosci. Biotechnol. Biochem. 66 (2002) 2297). In the current study, we cloned a genomic DNA frag...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 161(2004), 11 vom: 02. Nov., Seite 1277-9
1. Verfasser: Yoshida, Midori (VerfasserIn)
Weitere Verfasser: Lin, Dingbo, Kawakami, Akira
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article DNA, Complementary DNA, Plant Hexosyltransferases EC 2.4.1.- inulosucrase EC 2.4.1.9
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520 |a We previously reported the cloning of a wheat sucrose:sucrose 1-fructosyltransferase (1-SST) cDNA, designated wft2. Wft2 proteins have fructosyltransferase enzyme activity and initiate fructan synthesis (Biosci. Biotechnol. Biochem. 66 (2002) 2297). In the current study, we cloned a genomic DNA fragment carrying the full-length 1-SST gene from winter wheat (Triticum aestivum). The genomic 1-SST gene is 3326 bp in length and contains four exons and three introns. Exon 2 has only 9 bp. This sequence encodes a part of a beta-fructosidase motif (NDPNG), a highly conserved motif found in plant invertases. This is the first report of a mini exon, one of the smallest exons known in plants, being found in a genomic 1-SST gene 
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