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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ramgareeb, Sumita
|e verfasserin
|4 aut
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|a Responses of meristematic callus cells of two Cynodon dactylon genotypes to aluminium
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|c 2004
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 07.01.2005
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Responses to Al3+ of embryogenic callus cells of an Al-sensitive (Al-S) and Al-resistant (Al-R) Cynodon dactylon genotype were evaluated with regard to Al3+ toxicity and resistance. A chemical equilibrium speciation model (MINTEQA2) was used to ensure the availability of the Al3+ ion in culture media, which was supplied as 0.08-2.3 mM Al3+ for 2-8 weeks. Increasing Al3+ concentration and exposure time had a greater negative impact on the Al-S than on the Al-R genotype, in terms of callus growth rate and frequency of non-embryogenic cells. Exposure to 0.8 mM Al3+ for 2 weeks resulted in an 88% reduction in the Al-S meristematic cell number, whereas that of the Al-R genotype remained unaffected. In addition, the Al-S cells accumulated three times more Al in the nucleus than did the Al-R cells, suggesting that Al interfered with mitosis. The Al-R cells appeared to exclude Al3+ from its cells through an increase in extracellular pH (4.34 in Al-R and 4.08 in Al-S) and by the immobilisation of Al in the cell wall (33% more in Al-R). The results showed that by studying the cellular responses to Al3+ it is possible to discriminate between the Al-S and Al-R C. dactylon genotypes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Citric Acid
|2 NLM
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|a 2968PHW8QP
|2 NLM
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|a Aluminum
|2 NLM
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|a CPD4NFA903
|2 NLM
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|a Cooke, John A
|e verfasserin
|4 aut
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|a Watt, M Paula
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 161(2004), 11 vom: 02. Nov., Seite 1245-58
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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|g volume:161
|g year:2004
|g number:11
|g day:02
|g month:11
|g pages:1245-58
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