Effects of cadmium and copper on peroxidase, NADH oxidase and IAA oxidase activities in cell wall, soluble and microsomal membrane fractions of pea roots

Twelve-day-old seedlings of pea (Pisum sativum L.) that were treated for 4 days by 20 and 100 micromol/l Cd(NO3)2 or CuSO4 showed a growth reduction in all organs. From root protein extracts, the activities of guaiacol peroxidase (GPX; EC 1.11.1.7), ascorbate peroxidase (APX; EC 1.11.1.11), conifery...

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Veröffentlicht in:Journal of plant physiology. - 1979. - 161(2004), 11 vom: 10. Nov., Seite 1225-34
1. Verfasser: Chaoui, Abdelilah (VerfasserIn)
Weitere Verfasser: Jarrar, Brahim, El Ferjani, Ezzedine
Format: Aufsatz
Sprache:English
Veröffentlicht: 2004
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Cadmium Compounds Multienzyme Complexes Nitrates indoleacetic acid oxidase EC 1.11.- Peroxidases EC 1.11.1.- NADH oxidase EC 1.6.- mehr... NADH, NADPH Oxidoreductases Copper Sulfate LRX7AJ16DT cadmium nitrate VF9RQV8VXV
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245 1 0 |a Effects of cadmium and copper on peroxidase, NADH oxidase and IAA oxidase activities in cell wall, soluble and microsomal membrane fractions of pea roots 
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520 |a Twelve-day-old seedlings of pea (Pisum sativum L.) that were treated for 4 days by 20 and 100 micromol/l Cd(NO3)2 or CuSO4 showed a growth reduction in all organs. From root protein extracts, the activities of guaiacol peroxidase (GPX; EC 1.11.1.7), ascorbate peroxidase (APX; EC 1.11.1.11), coniferyl alcohol peroxidase (CAPX), NADH oxidase, and indole-3-acetic acid (IAA) oxidase were measured in covalently--and ionically--[symbol: see text] bound cell wall, soluble, and microsomal membrane fractions. With the exception of 20 micromol/l Cu, metal treatments enhanced GPX activity in all fractions. Only IAA oxidase activity was metal-elevated in the covalently bound cell wall fraction, while the ionic one showed Cd stimulation for all assayed enzymic activities. These effects were not entirely observed in Cu-treated plants, since APX and IAA oxidase activities were only enhanced in this fraction. However, soluble extract showed stimulation of APX activity, while in the microsomal fraction metal exposure also increased the activities of CAPX and NADH oxidase. Differential responses of root cell fractions to the presence of cadmium and copper ions are discussed in regard to the contribution of their enzymic capacities in antioxidant, lignification, and auxin degradation pathways. Comparisons between metals and dose effects are also underlined 
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650 7 |a Nitrates  |2 NLM 
650 7 |a indoleacetic acid oxidase  |2 NLM 
650 7 |a EC 1.11.-  |2 NLM 
650 7 |a Peroxidases  |2 NLM 
650 7 |a EC 1.11.1.-  |2 NLM 
650 7 |a NADH oxidase  |2 NLM 
650 7 |a EC 1.6.-  |2 NLM 
650 7 |a NADH, NADPH Oxidoreductases  |2 NLM 
650 7 |a EC 1.6.-  |2 NLM 
650 7 |a Copper Sulfate  |2 NLM 
650 7 |a LRX7AJ16DT  |2 NLM 
650 7 |a cadmium nitrate  |2 NLM 
650 7 |a VF9RQV8VXV  |2 NLM 
700 1 |a Jarrar, Brahim  |e verfasserin  |4 aut 
700 1 |a El Ferjani, Ezzedine  |e verfasserin  |4 aut 
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