Noble-metal nanoparticles directly conjugated to globular proteins
We report the synthesis of gold nanoparticles directly conjugated to bovine serum albumin protein by chemical reduction in aqueous solution. Transmission electron microscopy reveals that the gold nanoparticles are well dispersed with an average diameter less than 2 nm, and elemental analysis verifie...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 20(2004), 26 vom: 21. Dez., Seite 11778-83 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2004
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Serum Albumin Gold 7440-57-5 |
| Zusammenfassung: | We report the synthesis of gold nanoparticles directly conjugated to bovine serum albumin protein by chemical reduction in aqueous solution. Transmission electron microscopy reveals that the gold nanoparticles are well dispersed with an average diameter less than 2 nm, and elemental analysis verifies the composition of the gold-protein conjugates. Infrared spectroscopy confirms that the polypeptide backbone is not cleaved during the conjugation process and that the side chain functional groups remain intact. Raman spectroscopy demonstrates that the disulfide bonds in the conjugated protein are broken and thus are available for interaction with the nanoparticle surface. This synthesis method is a new technique for directly attaching gold nanoparticles to macromolecular proteins |
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| Beschreibung: | Date Completed 03.02.2006 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 0743-7463 |